人促甲状腺激素受体的制备及其敏感性测定

Preparationand Sensitivity Measure of Human Thyrotropin (TSH) Receptor

关键词:  甲状腺  受体，促甲状腺激素  G蛋白  腺苷酸环化酶  人

摘要:

Objective　To explore a simple and economical experimental method that can extract human thyroid TSH receptor-G_s protein-Adenylate cycles (AC) signal pathway and measure the sensitivity of TSH receptor.Methods　TSH receptor in cell membraone proteins and G_s protein-AC that coupled with TSH receptor was extracted from human thyroid preserved in low temperature by highspeed centrifugation.The sensitivity of TSH receptor combined with ligand human TSH (hTSH) was presented as the activity of AC.Results　The amount of membrane protein preparation (30～180 μg) was significantly related to the amount of cAMP produced (r=0.987,P＜0.05), the concentration of ligand hTSH (0～2 500 μ IU/ml) and G_s protein stimulator GTP γ S (0～1.0 mmol/L) were highly significantly related to the amount of cAMP produced (r=0.954,P＜0.01 and r=0.986,P＜0.001).Conclusion　The perfect TSH receptor-G_s protein-AC signal pathway was obtained by this method, and the activity of AC can perfectly stand for the sensitivity of TSH receptor combined with ligand hTSH, this simple and economic method can be used in experimental study in thyroid TSH receptor-G_s protein-AC signal pathway.

Abstract: