miR-373抑制剂对乳腺癌细胞生长的影响

Effects of miR-373 inhibitor on growth of breast cancer cells

关键词:  乳腺肿瘤  微小RNA  抑制剂  细胞增殖

Key words:  breast cancer  microRNA  inhibitor  cell proliferation

摘要:

目的 探索miR-373抑制剂对乳腺癌细胞系MCF-7生长的影响。方法 设计并合成miR-373抑制剂,通过脂质体转染至MCF-7细胞,采用荧光定量聚合酶链式反应检测转染后细胞内miR-373的表达水平,同时检测Caspase-3与Caspase-8的表达情况,并应用MTT实验检测miR-373抑制剂对MCF-7细胞生长的影响。结果 miR-373抑制剂能有效抑制MCF-7细胞中miR-373的表达。转染后,MCF-7细胞中Caspase-3与Caspase-8的mRNA水平均升高（P＜0.05）。miR-373抑制剂对MCF-7细胞的生长有明显的抑制作用,抑制率随浓度升高而增加（P＜0.05）。结论 miR-373抑制物可有效抑制MCF-7细胞增殖,有望成为治疗乳腺癌的新靶点。

Abstract:

Objective To investigate the effects of miR-373 inhibitor on growth of breast cancer cell line MCF-7.Methods The miR-373 inhibitor was designed,synthesized and transfected into MCF-7 cells.The expression of miR-373,Caspase-3 and Caspase-8 were detected by quantitative real-time polymerase chain reaction.The cell proliferation was analyzed through MTT assay.Results miR-373 inhibitor inhibited the expression of miR-373 efficiently.The expression of Caspase-3 and Caspase-8 were up-regulated after MCF-7 cells were transfected with miR-373 inhibitor(P＜0.05 ).miR-373 inhibitor inhibited proliferation of MCF-7 cells significantly and the inhibition rate increased with the concentration of miR-373 inhibitor.Conclusion The miR-373 inhibitor can efficiently inhibit the proliferation of breast cancer MCF-7 cells,which may provide a new strategy for the target therapy of breast cancer.