胃食管反流病患者食管黏膜Ki-67及Caspase-3的表达

Expression of Ki-67 and Caspase-3 in esophageal mucosa of patient with gastroesophageal reflux disease

关键词:  Ki-67  Caspase-3  胃食管反流病

Key words:  Ki-67  Caspase-3  GRED

摘要:

目的探讨胃食管反流病(gastroesophageal reflux disease,GERD)患者食管黏膜组织中Ki-67、Caspase-3的表达及意义。方法收集非糜烂性食管炎(non-erosive esophageal reflux disease,NERD)患者、反流性食管炎(reflux esophagitis,RE)患者、Barrett食管患者食管黏膜各20例,正常食管黏膜组织15例作为对照,分别对食管黏膜组织进行常规病理检查、免疫组化及western-blot分析Ki-67、Caspase-3的表达。结果GERD的各组中均存在食管黏膜组织的明显增生.NERD组、RE组、BE组的食管黏膜Ki-67阳性表达率明显高于正常对照组(分别是80%、85%、95%及6.7%,P＜0.05)；在NERD组、RE组、BE组,食管黏膜Caspase-3阳性表达率分别是95%、90%和75%,仅BE组与正常对照组100％比较,差异有统计学意义(P＜0.05)。结论食管黏膜组织中的ki-67、Caspase-3的表达,在一定程度上反映了食管黏膜鳞状上皮细胞的增殖程度,与食管黏膜的组织抵抗力的改变有关,Ki-67和Caspase-3可能同时共同参与了食管癌的发生。

Abstract:

Objective To investigate the expression and significance of Ki-67 and Caspase-3 in the esophageal mucosa of patients with gastroesophageal reflux disease(GERD).Methods Sixty GERD patients,including 20 cases of reflux esophagitis(RE),20 cases of non-erosive reflux disease (NERD) and 20 cases of Barrett esophagus(BE) were enrolled in the study;esophageal mucosa samples were also taken from 15 normal healthy subjects as controls.The esophageal samples were examined with routine histopathology;the expressions of Ki-67 and Caspase-3 proteins in the esophageal mucosa were detected by immunohistochemistry and Western-blot methods.Results Prominent proliferation in esophageal mucous membrane was observed histopathologically in all 3 groups of GERD patients.The positive rates of Ki-67 and intensity of expression in NERD,RE and BE groups were 80%,85%and 95%,which were significantly higher than that of controls(6.7%, P＜0.05);while the expression of Caspase-3 in NERD,RE,and BE groups were 95%,90%and 75%,only BE group were lower than control group(100.0%,PP＜0.05).Conclusion Expression of Ki-67 and Caspase-3 may be associated with the proliferation of esophageal mucous membrane and the change of tissue resistance in gastroesophageal reflux disease.