NF-κB和STAT3在早期肾癌中的表达及临床意义

Expression of NF-κB and STAT3 in early stage renal cell carcinoma and their clinic significance

关键词:  核因子KB  信号转导与转录活化因子3  肾细胞肿瘤  免疫组织化学

Key words:  renal cell carcinoma  nuclear factor-κB  signal transducfion activating transcription 3  immunohistochemistry

摘要:

目的检测核因子KB（nuclearfactor-κB，NF-κB）和信号转导与转录活化因子3（signaltransducerandactivatoroftranscription3，STAT3）在早期肾癌组织中的表达情况，探讨两者在肾癌发生发展中的意义。方法运用免疫组织化学方法检测62例早期肾癌组织和20例癌旁组织标本中NF-κB和STAT3及其磷酸化产物P-NF-κB和P-STAT3的阳性表达情况，分析其与临床、病理参数的相关性；运用聚合酶链技术检测20对肾癌和癌旁组织中NF-κB和STAT3mRNA表达情况，并行统计分析。结果免疫组化结果显示NF-κB、p-NF-κB和P-STAT3在肾癌组织中的表达率分别为62.9％（39/62）、58.1%（36/62）和50.0%（31/62），明显高于癌旁组织的10%（2/20）、0%（0/20）和15%（3/20），差异有统计学差异（P＜0.05）。NF-κB和P-NF-κB的表达与临床分期、肿瘤病理分级有相关性。聚合酶链反应（PCR）结果显示肾癌组织中NF-κB mRNA表达率（55.9%）较癌旁组（32.7%）高，差异有统计学意义（P＜0.01）；肾癌组织中STAT3mRNA表达率（55.3％）较癌旁组（49.7％）高，但差异无统计学意义（P＞0.05）。结论NF-κB和STAT3均有望成为肾癌的诊断标志物和治疗靶点，且前者意义更大。

Abstract:

Objective To investigate the expression and the clinic significance of nuclear factor-κB （NF-κB） and signal transducer and activator of transcription 3 （STAT3） in early stage renal cell carcinoma （RCC）. Methods The expression of NF-κB and STAT3 protein was determined by immunohistochemistry in 62 samples of RCC tissues and 20 samples of pericancerous tissue. The mRNA expression of NF-κB and STAT3 was detected by RT-PCR in 20 samples of RCC tissues and pericancerous tissue. The correlation of NF-κB and STAT3 expression with clinical and pathological parameters were analyzed. Statistical analyses were performed with SPSS version 13. 0 software. Results NF-κB, p-NF-κB and p-STAT3 in renal cell carcinoma （62.9%, 58.1% and 50.0%） was significantly higher than those in pericancerous tissue （10%, 0% and 15%） （P＜0.05）. Theoverexpression of NF-κB and p-NF-κB was closely correlated with clinical stage and histological grade （P＜0.05）. The positive expression rate of NF-κB mRNA in RCC was significantly higher than that in pericancerous tissue （55.9% vs 32.7%, P=0.0015） ; while there was no significant difference in the expression of STAT3 mRNA between two groups （P＞0.05）. Conclusion NF-KB and STAT3 may be of value in early diagnoses and treatment of RCC.