FAN Jian,YU Guang-rong,YUAN Feng,et al.Expression of glucose transporter protein-1 in benign and malignant bone tumors and its clinical significance[J].Journal of Tongji University(Medical Science),2013,34(3):28-31. [点击复制]
Objective To investigate the expression of glucose transporter protein-1（Glut-1） in normal bone tissue,benign and malignant bone tumors and its clinical significance.Methods Immunohistochemical staining was applied to detect Glut-1 expression in 32 cases of bone osteosarcoma,10 cases of osteochondroma and 10 specimens of normal bone tissue.The expression of Glut-1 protein was also examined by Wester-blotting method in fresh specimens of osteosarcoma and normal bone tissue.Results Immunohistochemistry showed positive expression of Glut-1 in 31cases of osteosarcoma（96.9%）,including strongly positive in 20 cases（62.5%） and weakly positive in 1 case.All osteochondroma specimens presented negative staining for Glut-1,except one case presenting weakly positive,while no positive expression of Glut-1 was detected in normal bone tissue.Western-blot showed the gray value increased significantly in 3 fresh specimens of osteosarcoma compared to normal bone tissue（P＜0.05）.Conclusion Glut-1 protein is positively expressed in osteosarcoma,which indicates that Glut-1 may be used to assess malignancy of bone tumors and as a potential target for treatment of bone tumors.
