DONG Xiao-yan,ZHANG Rong,CHEN Xin-liang.Effect of lentivirus-mediated LOX RNA interference on expression of elastin and matrix metalloproteinase-2 in human vaginal wall fibroblasts[J].Journal of Tongji University(Medical Science),2016,37(4):19-24, 30. [点击复制]
Effect of lentivirus-mediated LOX RNA interference on expression of elastin and matrix metalloproteinase-2 in human vaginal wall fibroblasts
DONG Xiao-yan,ZHANG Rong,CHEN Xin-liang
(Dept.of Obstetrics and Gynecology, Karamay Central Hospital, Karamay 834000, Xinjiang Uygur Autonomous Region, China;Dept.of Gynecology, the International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China)
Abstract:
Objective To investigate the effect of lentivirus-mediated lysine oxidase (LOX) RNA interference on expression of elastic protein and matrix metalloproteinase-2 (MMP-2) in human vaginal wall fibroblasts. Methods Anterior vaginal tissue samples were collected from healthy women before and after menopause, the fibroblasts were isolated from vagina tissue by enzymes digestion. The cultured cells were identified using immunofluorescence method. Four lentivirus RNAi vectors of LOX (LOX-RNAi-LV) were constructed and transfected to HEK-293T cells to screening the most efficient LOX-RNAi-LV. The screened LOX-RNAi-LV was transfected to human vaginal tissue-derived fibroblasts cells. The mRNA expression of elastin and MMP-2 was detected by RT-PCR. Results The LOX-RNAi-LV with high efficiency was successfully constructed and transfected to fibroblasts isolated from anterior vaginal wall. The mRNA expression of elastin was decreased and MMP-2 was increased, compared to those before transfection. Conclusion Results indicate that LOX may have a regulatory effect on the expression of elastin and matrix metalloproteinase 2 in fibroblasts of vagina tissue, and may be involved in pelvic organ prolapse.
