高频超声心动图评价IGFBP-4转基因小鼠心肌梗死后心脏功能的变化

Assessment of cardiac function of IGFBP-4 transgenic mice with myocardial infarction by high-frequency echocardiography

关键词:  高频超声心动图  胰岛素样生长因子结合蛋白-4  小鼠  心肌梗死

Key words:  high-frequency echocardiography  insulin-like growth factor binding protein-4  mice  myocardial infarction

摘要:

目的 探讨高频超声心动图在胰岛素样生长因子结合蛋白-4（insulin-like growth factor binding protein-4, IGFBP-4）转基因小鼠心肌梗死后心脏功能评估中的作用。方法 实验分为对照组（n=27）和IGFBP-4转基因组（n=27）。通过结扎冠状动脉左前降支建立心肌梗死模型，于模型建立前，建立后1、2周分别使用高频超声心动图进行心功能评估，比较两组心肌梗死前后心功能变化差异。2周后处死小鼠进行病理学检查，并称取左心室质量，与高频超声左心室质量测量值进行相关性检验。 结果 结扎术后，超声心动图检查发现两组小鼠心功能较术前明显降低，左心室射血分数（ejection fraction, EF）及左心室短轴缩短率（fractional shortening, FS）较术前降低（均P<0.05），左心室舒张末直径及收缩末直径较术前增大（均P<0.05）；术后2周，超声心动图检查发现IGFBP-4转基因组小鼠EF及FS值高于对照组（P<0.05）,左心室舒张末直径及收缩末直径大于对照组（P<0.05）。超声心动图测得左心室质量与尸解左心室质量实际值相关性较好，对照组及IGFBP-4转基因组r值别为0.89与0.93。结论 高频超声心动图能够用于准确评价IGFBP-4转基因小鼠心肌梗死模型心脏功能的变化。

Abstract:

Objective To assess the cardiac function of insulin-like growth factor binding protein-4 (IGFBP-4) transgenic mice with myocardial infarction by high-frequency echocardiography. Methods The myocardial infarction was induced by ligation of left anterior descending coronary artery in ICR male mice (control group, n=30) and IGFBP-4 transgenic mice (n=30). The cardiac function was assessed by high-frequency echocardiography before and 1, 2 weeks after modeling. The mice were sacrificed for pathological examination and left ventricular mass (LVM) weighing. The relevance of LVM value measured by high-frequency echocardiography and that of postmortem examination was analyzed. Results Echocardiography showed that (ejection fraction)EF and (fractional shortening)FS value decreased (P<0.05) while (left ventricular internal diameter at end diastolic)LVIDd and (left ventricular internal diameter at end systolic)LVIDs increased markedly (P<0.05) after modeling. And the reduction of EF and FS value in IGFBP-4 transgenic mice was significantly lower than that in the control group（P<0.05）; the enlargement of LVIDd and LVIDs was also lower than that of control group（P<0.05）. The LVM value measured by high-frequency echocardiography was closely correlated with that examined by postmortem both in control group (r=0.89, P<0.01) and in IGFBP4 group (r=0.93, P<0.01). Conclusion The cardiac function change of IGFBP-4 transgenic mice group can be accurately assessed by high-frequency echocardiography.