可注射型富血小板纤维蛋白促进人脐静脉内皮细胞成血管能力的体外实验研究

Effect of injectable platelet-rich fibrin on angiogenesis of human umbilical vein endothelial cells in vitro

摘要:

目的 探究可注射型富血小板纤维蛋白(injectable platelet-rich fibrin, iPRF)对体外培养的人脐静脉内皮细胞(human umbilical vein endothelial cells, HUVECs)成血管分化能力的影响。方法 CCK-8实验检测HUVECs增殖能力并筛选最佳浓度的可注射型富血小板纤维蛋白提取液(injectable platelet-rich fibrin extract, iPRFe)；划痕实验和小管形成实验分别检测HUVECs迁移能力和成血管分化能力；实时定量PCR检测成血管相关基因的mRNA表达。结果 0.4 mg/mL浓度的iPRFe促进HUVECs增殖效果最佳；iPRFe对HUVECs迁移和小管形成有显著的促进作用，还能明显提高血管生成素(angiogenin, ANG)、C-X-C基序趋化因子受体4(C-X-C motif chemokine receptor 4, CXCR4)、血小板衍生生长因子受体A(platelet-derived growth factor receptor A, PDGFRA)和血管内皮生长因子(vascular endothelial growth factor, VEGF)的表达水平。结论 iPRF可以促进HUVECs增殖、迁移和成血管能力，有望成为用于牙髓再生的新材料。

Abstract:

Objective To explore the effect of injectable platelet-rich fibrin(iPRF) on angiogenesis of human umbilical vein endothelial cells(HUVECs) in vitro. Methods The proliferation ability of HUVECs was detected by the Cell Counting Kit-8(CCK-8) and the optimal concentration of iPRF extract(iPRFe) was selected. The migration ability and angiogenesis capacity of HUVECs were detected by scratch assay and tube formation assay, respectively. Real-time quantitative polymerase chain reaction(RT-qPCR) was performed to evaluate the mRNA expressions of angiogenic genes. Results The iPRFe at 0.4 mg/mL had the best effect on the proliferation of HUVECs. The iPRFe significantly promoted migration and tube formation of HUVECs, and significantly increased the expressions of angiogenin(ANG), C-X-C motif chemokine receptor 4(CXCR4), platelet-derived growth factor receptor A(PDGFRA), and vascular endothelial growth factor(VEGF). Conclusion The iPRF can promote the proliferation, migration and angiogenesis of HUVECs, and may be used as a novel material for pulp regeneration.