| 摘要: |
| 本文扼要叙述。显微分光扫描法的基本原理,结合计算机数据处理给出了一些细胞基本参数诸如细胞核,质面积;细胞内物质吸收光谱、相对含量;细胞二维灰度图、三维含量分布图等。列举过热BGC胃癌细胞SDH含量及正常小白鼠肝细胞核内DNA含量测定,说明该测量法有较好的可靠性、重复性。并初步探讨使用该方法可能产生误差及克服误差方法等问题。 |
| 关键词: 扫描光径,三维含量分布,柯勒照明 |
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| SCANNING MICROPHOTOMETRY AND ITS APPLICATION ON INTRACELLULAR QUANTITATIVE ANALYSIS |
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| Abstract: |
| The report outlined the basic principles of scanning microphotometry, an approach on intracellular quantitative analysis. With the aid of a microcom- puter, basic information such as area of nucleus plasma, absorption spectrum of the measured object, tota |
| Key words: Scanning microphotometry,Three-dimensional content representation,kohler illumination |