| 摘要: |
| 目的：检测tubulin与chs rex b同源基因在左眼视损毁后鸽脑不同脑区的差异表达，方法：以经消减抑制杂交（suppression subtraction hybridization SSH)方法获得的鸽脑差异表达基因的表达序列标签（expressed sequence tag,EST)为探针，用放射性同位素对其进行标记，藉Northern杂交法分析其mRNA在不同脑区的丰度，结果；与人tubulin有高度同源性及与鸡的chs-rex-b m,RNA有高度同源性的基因 左，右视盖的丰度明显高于左，右前脑，结论：tubulin与rex b同源基因在鸽脑视觉相关脑区的发育过程中可能具有某此独特作用。 |
| 关键词: 鸽 表达序列标签 Northern杂交 前脑 视盖 单眼视觉剥夺 视通路 tubulin chs－rex－b 同源基因 |
| DOI： |
| 通信作者: |
| 修订日期：2001-03-01 |
| 录用日期： |
| 基金项目:国家自然科学基金资助项目（39770244） |
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| Expressions of Tubulin andChs Rex b Homologous Gene in Different Brain Regions of Monocular Eye Deprived Pigeon |
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| Abstract: |
| Objective To observe the expression of tubulin and chs rex b homologous gene in different region of monocular eye deprived pigeon.Methods The differential expressed gene in the pigeon brain was used as probe and labeled with radioisotope.The expressions |
| Key words: pigeon,expressed sequence tags,northern hybridization,forebrain,optic tecta |
