| 摘要: |
| 目的：探讨联合应用粉防已碱（tetrandrine,Tet）与甘草酸（glycyrrhizinic acid,Glz）对肝星状细胞增殖与细胞外基质合成的影响。方法：采用胶原酶二步原位灌注法分离、培养大鼠HSC，并分别给予2．5mg/L及5mg/L Tet、0．5mg/L Glz、2．5mg/L Tet 0.5mg/L Glz、5mg/L Tet 0.5mg Glz干预，分别检测各处理组HSC增殖及细胞外基质合成水平。结果：5mg/L Tet、0.5mg/L Glz和各浓度Tet联合Glz均能不同程度地抑制HSC 3H－TdR掺入和增殖（P＜0．05）；能够显著抑制体外培养HSC 3H－脯氨酸掺入（P＜0．05），降低上清液HA、Ln和CIV水平。5mg/L Tet联合Glz作用最为显著（P＜0．05）。结论：联合应用Tet与Glz能够更加有效地抑制HSC DNA合成、细胞增殖及ECM合成与分泌。 |
| 关键词: 粉防已碱 甘草酸 肝星状细胞 增殖 细胞外基质 |
| DOI： |
| 通信作者: |
| 修订日期：2001-12-28 |
| 录用日期： |
| 基金项目:上海市科学技术发展基金资助项目 ( 984310 33) |
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| Effect of Combination of Tetrandrine and Glycyrrhizinic Acid on Proliferation and Extracellular Matrix Synthesis of Hepatic Stellate Cells |
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| Abstract: |
| Objective To investigate the effects of combination of tetrandrine and glycyrrhizinic acid on the proliferation and extracellular matrix synthesis of hepatic stellate cells.Methods Hepatic stellate cells were isolated from normal male Sprague Dawley ra |
| Key words: tetrandrine,glycyrrhizinic acid,hepatic stellate cells,proliferation,extracellular matrix |
