| 引用本文： |
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傅锦业,范跃祖,赵泽明,陈春球.去甲斑蝥素对人胆囊癌GBC-SD细胞系增殖相关基因蛋白PCNA、Ki-67的影响[J].同济大学学报（医学版）,2003,(5):374-377. [点击复制]
- .Influence of Norcantharidin on the Expression of Proliferationrelated Gene Proteins:PCNA and Ki-67 of GBC-SD Cells in Human Gallbladder Carcinomas[J].Journal of Tongji University(Medical Science),2003,(5):374-377. [点击复制]
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| 摘要: |
| 目的 探讨去甲斑蝥素对人胆囊癌GBC SD细胞系增殖相关基因蛋白PCNA、Ki 67的影响。方法 应用体外细胞培养技术进行人胆囊癌GBC SD细胞的培养和传代 ;将去甲斑蝥素作用于经体外培养的人胆囊癌GBC SD细胞 ,应用SABC法检测其对GBC SD细胞增殖相关基因蛋白PCNA和Ki 67表达影响。结果 对照组GBC SD细胞可见核仁或胞核呈不同程度的PCNA或Ki 67棕褐染色 ,PCNA和Ki 67阳性指数分别达 0 .93 2和 0 .964;去甲斑蝥素 (60 μg/ml)作用 48h后 ,PCNA表达和Ki 67表达阳性的细胞数明显减少 ,PCNA和Ki 67阳性指数仅为 0 .3 18和 0 .2 97,差异有显著性 (P <0 .0 5)。结论 去甲斑蝥素可影响GBC SD细胞增殖相关基因蛋白PCNA和Ki 67的表达 ,这可能是其抑制人原发性胆囊癌GBC SD细胞增殖的机制之一 |
| 关键词: 胆囊肿瘤 细胞培养 去甲斑蝥素 PCNA Ki-67 |
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| 修订日期：2003-03-29 |
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| Influence of Norcantharidin on the Expression of Proliferationrelated Gene Proteins:PCNA and Ki-67 of GBC-SD Cells in Human Gallbladder Carcinomas |
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| Abstract: |
| Objective To study the influence of norcantha ri din on the expressions of proliferation-related gene protein:PCNA and Ki-67 of GBC-SD cell lines of human gallbladder carcinoma in vitro.Methods GBC-SD cell lines of human gallbladder carcinoma were cultu |
| Key words: gallbladder neoplasm,cell culture,norcantha ridin,PCNA,Ki-67 |
