多囊卵巢综合征卵巢组织与血清中VEGF、ES及MVD的表达与意义

Correlation of VEGF, ES expression and tissue MVD with sexual hormones levels in polycystic ovary syndrome

关键词:  多囊卵巢综合征  血管内皮生长因子  内皮抑素  微血管密度  免疫组化

Key words:  polycystic ovary syndrome  vascular endothelial growth factor  endostatin  microvessel density  immunity organism chemistry

摘要:

目的 研究多囊卵巢综合征（polycystic ovary syndrome,PCOS）卵巢组织及血清中VEGF、ES、MVD的表达,探讨VEGF、ES、MVD在PCOS的发生发展中的作用。方法 用免疫组化SP法检测卵巢组织中VEGF及ES的表达,ELISA法检测血清中VEGF和ES含量;用CD34标记新生血管内皮细胞,观察微血管密度（microvasculardensity,MVD）。结果 PCOS患者卵巢组织中VEGF及MVD均显著高于正常对照组（P＜0.05）;PCOS组及对照组卵巢组织中的VEGF、MVD表达均呈显著正相关（P＜0.01）;PCOS组血清中VEGF与T呈两两正相关（r=0.809,P＜0.01）;PCOS患者血清中VEGF与FSH、E、LH无相关性（P＞0.05）。结论 VEGF在卵巢内高表达,同时VEGF与ES之间表达失衡,可能是PCOS的发病机制之一;VEGF可促进卵巢雄激素的合成与分泌。

Abstract:

Objective To investigate the correlation of vascular endothelial growth factor(VEGF), endostatin(ES) expression and tissue microvascular density(MVD) in women with polycystic ovary syndrome(PCOS).Methods The expression of VEGF and ES in the ovarian tissues was detected by immunohistochemistry SP method and the serum VEGF and ES were measured by enzyme linked immunosorbent assay(ELISA).The microvascular density(MVD) in ovarian tissue was assayed by staining of vascular endothelial cells with CD34.Results The expressions of VEGF,ES and MVD in ovarian tissues of PCOS patients were higher than those of healthy women(P＜0.05).The expression of VEGF was positively correlated with MVD in ovarian tissues both in PCOS patients and controls (P＜0.01).Serum VEGF was positively correlated with blood LH and T levels in PCOS patients,but not correlated with FSH,E and P levels.Conclusion The expression of VEGF is likely to associate with the pathogenesis of PCOS.