ZHU Yu-jie,SHEN Zheng-yu,ZHU Mei-qin,et al.Immune abnormalities mediated by co-stimulatory molecules in patients with psoriasis vulgaris[J].Journal of Tongji University(Medical Science),2012,33(2):28-32. [点击复制]
(1.Dept.of Dermatology,Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200011,China;2.Dept.of Dermatology,Shanghai Tangwai Hospital,Shanghai 201400,China;3.Shanghai Institute of Immunology,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China)
Abstract:
Objective To investigate the immune abnormalities in patients with psoriasis vulgaris.Methods Blood samples were collected from 10 untreated patients with psoriasis vulgaris and 10 healthy subjects,and peripheral blood monocytes(PBMCs) were isolated.Expressions of CD86,B7H1,Foxp3,T-bet and GATA-3 were detected by RT-PCR and serum IL-12,IFN-γ,IL-17,IL-2,IL-10,IL-9,IL-22,IL-6,IL-13,IL-4,IL-5,IL-1β and TNF-α were measured by flow cytometry.Results The expressions of Foxp3 and GATA3 in psoriasis patients was lower,but expression of T-bet was higher than those in normal controls(P＜0.05).Cytokine analysis indicated that TNF-α in psoriasis patients was significantly higher than that in normal controls(P<0.05).The expression of CD86 in psoriasis was 0.3636±0.1003,which was significantly higher than that in normal controls(0.1061±0.03883,P＜0.05);while the expression of B7H1 was lower than that in control group(0.06876±0.03145 vs 0.3401±0.1152)(P＜0.05).Conclusion Th1 subset and TNF-α levels in psoriasis increase,while Th2 and Treg subsets significantly reduce.The expression of co-stimulatory molecule CD86 in PBMCs of psoriasis patients increases;while inhibitory molecule B7H1 decreases,indicating that immune dysfunc-tion in psoriasis patients may be mediated by co-stimulating molecules of antigen presenting cells(APCs).
