急性阑尾炎细菌培养及药敏试验

Germiculture and drug sensitivity test in acute appendicitis

关键词:  急性阑尾炎  脓液  细菌培养  药敏试验

Key words:  acute appendicitis  pus  germiculture  drug sensitivity test

摘要:

目的 分析急性阑尾炎患者病原菌分布及耐药性,指导临床抗菌药物应用。方法 对196例急性阑尾炎患者行阑尾切除术,术后采用VTTEK-32细菌自动分析系统对脓液标本做细菌培养及药敏试验。结果 196例标本中有123例检出细菌,阳性率62.76%,分离到细菌145株,其中革兰阴性菌131株,革兰阳性菌14株。主要感染细菌为大肠埃希菌、铜绿假单胞菌、克雷白杆菌等。药敏试验结果显示:细菌主要对头孢呋辛、头孢他啶、亚胺培南等敏感。结论 急性阑尾炎患者感染细菌以肠杆菌为主,其中大肠埃希菌占首位。应加强临床病原菌的检测与药敏试验,减少或避免耐药菌株的出现和预防院内感染。

Abstract:

Objective To investigate the pathogens and their drug resistance in acute appendicitis.Methods Pus samples were taken from 196cases of acute appendicitis.VTTEK-32 auto analysis system was applied for bacterial culture and drug sensitive test.Results Pathogens were detected in 123cases with a positive rate of 62.76%.Total 145 strains of bacteria were isolated from the samples,in which 131 strains were Gram-negative bacilli and 14 strains were Gram-positives.Escherichia coli,P.Aeruginosa and Klebsiella were most common,which were sensitive to cefuroxime,ceftazidime and imipenem.Conclusion Dominant pathogens are intestinal bacilli with predominance of E.coli in patients with acute appendicitis.Pathogen detections and drug sensitivity tests should be performed in acute appendicitis to select the effective antibiotics.