| 引用本文： |
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瞿小英,李胜蓝,李卓权.基于近红外荧光蛋白的细菌体内成像研究[J].同济大学学报（医学版）,2013,34(2):40-42. [点击复制]
- QU Xiao-ying,LI Sheng-lan,LI Zhuo-quan.Establishment of bacteria imaging system based on expression of near-infrared fluorescent protein[J].Journal of Tongji University(Medical Science),2013,34(2):40-42. [点击复制]
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| 摘要: |
| 目的建立表达iRFP的细菌株并且进行体内和体外成像。方法合成iRFP基因序列，构建pPL-BV和pET-3a-iRFP质粒，转化质粒进入细菌，检测细菌激发和发射波长，分析细菌体外和体内荧光分子成像。结果转化含iRFP质粒的细菌的激发波长峰值为690nm，发射波长峰值为713nm，细菌可进行体外和体内荧光分子断层成像。结论本研究成功构建了表达iRFP的细菌，体内外成像可快速分析该细菌的数量变化和体内分布。 |
| 关键词: 近红外荧光蛋白 细菌 活体成像 荧光分子断层成像 |
| DOI：10.3969/j.issn1008-0392.2013.02.009 |
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| Establishment of bacteria imaging system based on expression of near-infrared fluorescent protein |
| QU Xiao-ying1,LI Sheng-lan2,LI Zhuo-quan2 |
| (1. Dept. of Emergency, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;2. Dep. of Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai 200092, China) |
| Abstract: |
| Objective To establish bacteria imaging system based on expression of near-infrared fluorescent protein （iRFP）. Methods iRFP gene was de novo synthesized. The pPL-BV and pET-3a- iRFP plasmids were constructed and co-transfected into bacteria BL21. The excitation and emission spectra of iRFP-transfected bacteria BL21 were measured. Fluorescence molecular imaging of bacteria in vitro and in vivo were performed. Results The excitation and emission wavelength of iRFP- transfected bacteria was 690 nm and 713 nm, respectively. Fluorescence molecular imaging of iRFP- transfected bacteria was successfully performed in vitro and in vivo. Conclusion A bacterial strain expressing iRFP has been successfully established, which can be used for rapid analysis of bacteria quantity and distribution by in vivo and in vitro imaging. |
| Key words: near-infrared fluorescent proteins bacteria in vivo imaging fluorescence molecular tomography |
