白藜芦醇下调CCR7表达对肺癌细胞转移活性的影响

Resveratrol down-regulates CCR7 expression in lung cancer cells and its relation to cell migration ability in vitro

关键词:  白藜芦醇  肺肿瘤  CCR7  次级淋巴组织趋化因子  转移

Key words:  resveratrol  lung cancer  CCR7  secondary lymphoid tissue chemokine  metastasis

摘要:

目的探讨白藜芦醇对CCR7表达的作用及其对肺癌细胞A549、95D转移活性的影响。方法A549、95D肺癌细胞经不同剂量的白藜芦醇处理后,通过流式细胞术、荧光细胞化学技术、Western印迹法等观察CCR7的蛋白表达水平的变化;MTT法测定白藜芦醇对细胞毒性作用;划痕实验、Transwell小室测定细胞的迁移能力改变。结果A549、95D细胞有不同水平CCR7表达,白藜芦醇处理后,两种肺癌细胞CCR7表达降低,以A549更为显著,呈时间、剂量依赖性;白藜芦醇作用24 h后划痕实验、Transwell小室结果表明白藜芦醇具有抑制细胞的水平迁移能力,也抑制次级淋巴组织趋化因子（secondary lymphoid tissue chemokine,SLC）对肺癌细胞的趋化作用。结论白藜芦醇下调CCR7的表达,可能参与抑制肿瘤细胞转移的过程。

Abstract:

Objective To investigate the effect of resveratrol on expression of CCR7 in lung cancer cells and its relation to cell migration ability in vitro. Methods Human lung cancer A549 and 95D cells were treated with different concentrations of resveratrol in vitro. Expression of CCR7 protein in lung cancer cells was assayed by flow cytometry, immunofluorescent cytochemistry and Western blotting. Cell toxicity was examined by MTT method. The cell migration and invasion were evaluated by Transwell assay and scratch assay. Results A549 and 95D cells expressed CCR7 in different levels. After resveratrol treatment, CCR7 expression in lung cancer cells decreased in a dose-and time-dependent manner, which was more significantly for A549 cells. Transwell and scratch assay showed that resveratrol inhibited cell horizontal migration, and also inhibited the secondary lymphoid tissue chemokine （SLC） induced-chemotaxis on lung cancer cells. Conclusion The results suggest that resveratrol down-regulates expression of CCR7 in lung cancer cells, which might be associated with the inhibition of tumor cell metastasis.