抑制NBS1表达对三阴性乳腺癌细胞MDA-MB-231增殖和凋亡的影响

NBS1 gene knockdown suppresses proliferation and promotes apoptosis ofhuman triple-negative breast cancer MDA-MB-231 cells

关键词:  三阴性乳腺肿瘤  NBS1  细胞增殖  细胞凋亡  siRNA

Key words:  triple negative breast tumour  NBS1  cell proliferation  cell apoptosis  siRNA

摘要:

目的 探讨siRNA抑制NBS1基因表达对三阴性乳腺癌细胞MDA-MB-231增殖和凋亡的影响。方法通过构建NBS1干扰质粒和阴性空载质粒,并通过腺病毒载体感染MDA-MB-231细胞,Western印迹法明确NBS1基因受干扰情况；CCK-8法检测转染质粒后对MDA-MB-231细胞体外增殖能力的影响；流式细胞法检测感染后实验组和阴性对照组MDA-MB-231细胞的凋亡情况。结果 Western印迹法检测结果表明实验组NBS1蛋白表达水平明显低于阴性对照组和空白对照组(P<0.05)；CCK-8实验结果显示,与阴性对照组和空白对照组相比,实验组细胞增殖能力受到抑制(P<0.05)；流式细胞仪检测结果显示,实验组较阴性对照组凋亡率上升(P<0.05)。结论抑制NBS1基因的表达可以抑制三阴性乳腺癌细胞增殖,并促进癌细胞的凋亡。

Abstract:

Objective To investigate the effect of NBS1 knockdown on proliferation and apoptosis of human triple-negative breast cancer MDA-MB-231 cells. Methods MDA-MB-231 cells were infected with adenovirus vector containing NBS1-siRNA plasmid. The expression of NBS1 was determined by Western blotting. Cell proliferation was detected by CCK-8 assay, and cell apoptosis was analyzed by using FCM. Results Western blotting showed that the expression of NBS1 was decreased in NBS1-siRNAi transfected MDA-MB-231 cells (P<0.05). CCK-8 assay demonstrated that of the proliferation of MDA-MB-231 cells in NBS1-siRNA group was lower than that in negative and blank control groups (P<0.05). FCM revealed that the apoptosis rate of NBS1-siRNA group was higher than that of negative and blank control groups (P<0.05). Conclusion Inhibition of NBS1 gene expression can suppress the proliferation and promote apoptosis of human triple-negative breast cancer MDA-MB-231 cells.