胎盘早剥患者外周血单个核细胞中NF-κB的表达研究

Expression of NF-κB and TNF-α in peripheral blood mononuclear cells of patients with placental abruption

关键词:  胎盘早剥  核因子-κB  肿瘤坏死因子-α

Key words:  placental abruption  nuclear factor kappa B  tumor necrosis factor-α

摘要:

目的 探讨外周血单个核细胞(peripheral blood mononuclear cells, PBMCs)中NF-κB活化与TNF-α表达在胎盘早剥中的意义。方法以62例胎盘早剥患者为研究对象、正常孕产妇62例为对照组,Western印迹法测定PBMCs中NF-κB蛋白表达水平,RT-PCR检测血清TNF-α mRNA值的变化,并记录新生儿Apgar评分,进行相关性分析。结果与对照组相比,胎盘早剥患者PBMCs NF-κB表达升高(P＜0.05),TNF-α水平升高(P＜0.05),两指标之间呈显著正相关(P＜0.01),与新生儿Apgar评分呈负相关(P＜0.05)。结论NF-κB和TNF-α参与了胎盘早剥病理生理过程,提示胎盘早剥与炎症反应密切相关。

Abstract:

Objective To investigate the expression of nuclear factor kappa B(NF-κB) in peripheral blood mononuclear cells(PBMCs) and tumor necrosis factor-α(TNF-α)in patients with placental abruption. MethodsThe study comprised 62 placental abruption patients and 62 healthy pregnant women. The expression of NF-κB in PBMCs was detected by Western blotting and TNF-α mRNA was detected by RT-PCR. The association between expression of NF-κB, TNF-α and Apgar scores was analyzed. ResultsCompared to the controls, the expression of NF-κB protein and TNF-α mRNA was significantly increased and Apgar scores were decreased in placental abruption patients(all P＜0.01). The expression of NF-κB was positively associated with TNF-α concentration and the Apgar scores were negatively correlated with the expression of NF-κB and TNF-α(P＜0.05). ConclusionPlacental abruption patients have increased NF-κB and TNF-α expression, which may be related to placental abruption and inflammation.