WANG Li,FAN Wen-bin,LV Li-xia,et al.Transdifferentiation of human umbilical cord mesenchymal stem cells into retinal pigment epithelial like cells[J].Journal of Tongji University(Medical Science),2015,36(5):1-7. [点击复制]
Transdifferentiation of human umbilical cord mesenchymal stem cells into retinal pigment epithelial like cells
WANG Li,FAN Wen-bin,LV Li-xia,LI Peng,TIAN Hai-bin,XU Guo-tong
(Tongji Eye Institute, Medical College, Tongji University, Shanghai 200092, China;Dept.of Regenerative Medicine, Medical College, Tongji University, Shanghai 200092, China;Stem Cell Research Center, Medical College, Tongji University, Shanghai 200092, China)
Abstract:
Objective To investigate the transdifferentiation of human umbilical cord mesenchymal stem cells(hUC-MSCc) into retinal pigment epithelial(RPE) cells. Methods MSC specific markers were identified by flow cytometry in hUC-MSCs, and adipogenesis, osteogenesis and chondrogenesis were confirmed by culturing hUC-MSCsin differentiation media. Sox2, Pax6, Rax, Six6, Nr2e1, Otx2, Lhx2, Crx, Mitf-A, Klf4 and c-Myc were co-infected into hUC-MSCs by lentivirus-mediated system. The hUC-MSCs derived RPE-like cells were characterized by detecting the expressions of specific markers by real-time quantitative-polymerase chain reaction(Q-PCR), immunostaining and Western blot. ResultshUC-MSCs were positive for CD29, CD44, CD73, CD90, CD105 and negative for CD11b, CD34, CD45, HLA-DR. After induction, hUC-MSCs were differentiate into adipocytes, osteoblasts and chondrocytes. Retina and RPE development related transcription factors directly transdifferentiated hUC-MSCs into RPE-like cells which expressed RPE specific markers ZO-1, RPE65, Bestrophin-1, CK8/18, Cralbp, Mertk, Tyrosinase and PEDF. Conclusion hUC-MSCs could be directly transdifferentiated into RPE-like cells by key transcription factors.
