| 引用本文： |
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宗姗姗,赵鑫,罗成凤,等.吲哚胺2,3双加氧酶对人绒毛膜癌JEG-3 细胞增殖及迁移能力的影响[J].同济大学学报（医学版）,2016,37(2):31-34. [点击复制]
- ZONG Shan-shan,ZHAO Xin,LUO Cheng-feng,et al.Effect of indoleamine 2,3-dioxygenase on proliferation and migration in JEG-3 cells[J].Journal of Tongji University(Medical Science),2016,37(2):31-34. [点击复制]
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| 摘要: |
| 目的 观察吲哚胺2,3双加氧酶(indoleamine 2,3-dioxygenase, IDO)对人绒毛膜癌JEG-3细胞体外增殖、迁移能力的影响。方法 通过IDO抑制剂(1-L-MT)及短发夹RNA基因干扰抑制IDO表达,利用MTS增殖实验、Transwell迁移试验检测对细胞增殖和迁移能力的影响。结果 基因敲减能明显降低IDO表达水平。1-L-MT和基因敲减的JEG-3细胞与对照组相比,细胞的增殖和迁移能力明显下降。结论 抑制IDO能够降低绒癌细胞系JEG-3细胞的增殖和迁移能力。 |
| 关键词: 绒毛膜肿瘤 吲哚胺2,3双加氧酶 JEG-3 细胞增殖 细胞迁移 |
| DOI：10.16118/j.1008-0392.2016.02.007 |
| 通信作者: |
| 投稿时间：2015-11-29 |
| 录用日期： |
| 基金项目:国家自然科学基金(81270703)；上海市科学技术委员会项目(14140900203) |
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| Effect of indoleamine 2,3-dioxygenase on proliferation and migration in JEG-3 cells |
| ZONG Shan-shan,ZHAO Xin,LUO Cheng-feng,LI Chun-qing,WANG Kai,ZHOU Qian |
| (Dept. of Obstetrics and Gynecology, First Maternity and Infant Hospital, Tongji University, Shanghai 200040, China) |
| Abstract: |
| Objective To investigate the effect of indoleamine 2,3-dioxygenase(IDO) on proliferation and migration of human choriocarcinama JEG-3 cells. Methods JEG-3 cell function was studied by IDO inhibitor(1-L-MT) or shRNA. The proliferation and migration of JEG-3 cells were determined by MTT assay and Transwell system, respectively. Results shRNA knockdown significantly decreased IDO expression. 1-L-MT and shRNA knockdown of IDO decreased the proliferation and migration of JEG-3 cells. Conclusion The inhibitory of IDO can suppress the proliferation and migration of trophoblast cells in vitro. |
| Key words: choriocarcinama IDO JEG-3 proliferation migration |
