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曹帆帆,徐莉敏,王莹,等.雷公藤红素对β淀粉样蛋白诱导SH-SY5Y细胞Tau蛋白异常磷酸化影响的研究[J].同济大学学报（医学版）,2016,37(4):36-39, 50. [点击复制]
- CAO Fan-fan,XU Li-min,WANG Ying,et al.Effects of Tripterine on Tau hyperphosphorylation induced by β-amyloid peptides in cultured SH-SY5Y cells[J].Journal of Tongji University(Medical Science),2016,37(4):36-39, 50. [点击复制]
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| 雷公藤红素对β淀粉样蛋白诱导SH-SY5Y细胞Tau蛋白异常磷酸化影响的研究 |
| 曹帆帆,徐莉敏,王莹,彭彬,张雪,张登海 |
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| (第二军医大学附属公利医院中法合作中心实验室,上海 200135;第二军医大学附属公利医院检验科,上海 200135) |
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| 摘要: |
| 观察雷公藤红素对β淀粉样蛋白142导致SHSY5Y细胞Tau蛋白异常磷酸化的影响及其可能机制。〖HT5”H〗方法〖HT5”K〗用 Aβ142刺激SHSY5Y细胞，建立Tau蛋白异常磷酸化的细胞模型。加入雷公藤红素干预，Western印迹法检测Aβ142导致Tau蛋白异常磷酸化的变化，同时检测pNFSymbolkA@B表达情况；siRNA法下调TLR4表达，Western印迹法检测Aβ142导致的Tau蛋白异常磷酸化的变化情况；同时观察下调TLR4后，Aβ142刺激对pNFSymbolkA@B表达的影响。〖HT5”H〗结果〖HT5”K〗Aβ142刺激SHSY5Y细胞，导致Tau蛋白Ser199/202、Ser396位点的磷酸化水平增加，而雷公藤红素能降低这两个位点磷酸化水平；Aβ142导致细胞pNFSymbolkA@B表达增加，雷公藤红素干预后pNF SymbolkA@B表达下调，且NFSymbolkA@B抑制剂BAY117082同样能降低Aβ142导致的Tau蛋白异常磷酸化；下调TLR4表达，Aβ142导致的Tau蛋白异常磷酸化及pNFSymbolkA@B表达下降。〖HT5”H〗结论〖HT5”K〗雷公藤红素降低Aβ142导致的SH SY5Y细胞Tau蛋白异常磷酸化可能与其抑制TLR4/NF SymbolkA@ B通路活性有关。 |
| 关键词: 雷公藤红素 β淀粉样蛋白 Tau蛋白 Toll样受体4 pNFSymbolkA@B |
| DOI：10.16118/j.1008-0392.2016.04.007 |
| 通信作者: |
| 投稿时间：2016-04-18 |
| 录用日期： |
| 基金项目:上海市浦东新区卫生系统优秀青年医学人才培养计划(PWRq2013-11)；上海市青年科技英才扬帆计划(15YF1410800) |
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| Effects of Tripterine on Tau hyperphosphorylation induced by β-amyloid peptides in cultured SH-SY5Y cells |
| CAO Fan-fan,XU Li-min,WANG Ying,PENG Bin,ZHANG Xue,ZHANG Deng-hai |
| (Sino-French Cooperative Central Lab, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China;Clinical laboratory, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China) |
| Abstract: |
| Objective To investigate the effects and mechanisms of tripterine on Tau hyperphospho rylation induced by β amyloid in SH SY5Y cells. Methods The effects of tripterine on the changes of Tau hyperphosphorylation and p NF SymbolkA@B induced by βamyloid in SHSY5Y cells were measured by Western blotting after TLR4 knocking down by siRNA. ResultsCompared with the control group, the expressions of Tau pSer199/202 and pSer396 were markedly increased after the induction of Aβ142, while the hyperphosphorylation of Tau pSer199/202 and pSer396 induced by Aβ142 was decreased by tripterine. The increasing of pNFSymbolkA@B expression induced by Aβ142was decreased after the treatment of tripterine, and NFSymbolkA@B inhibitor BAY117082 also decreased the hyperphosphorylation of Tau pSer199/202 and pSer396 induced by Aβ142. The hyperphosphorylation of Tau pSer199/202 and pSer396 and pNFSymbolkA@B expression induced by Aβ142 was decreased after knocking down TLR4. ConclusionDecreasing of Tau hyperphosphorylation by Tripterine is partially via inhibiting the TLR4/NFSymbolkA@B signaling pathway． |
| Key words: tripterine βamyloid Tau Toll like receptor 4 pNF SymbolkA@B |