人不同组织间充质干细胞免疫调控能力的比较

Comparison of immune regulatory properties of human mesenchymal stem cells derived from different tissues

关键词:  免疫调控  间充质干细胞  单个核细胞  吲哚胺-2,3-双加氧酶  趋化因子

Key words:  immunoregulation  mesenchymal stem cells  peripheral blood mononuclear cells  indoleamine-2,3-dioxygenase  chemokines

摘要:

目的 比较人5种不同组织来源间充质干细胞(mesenchymal stem cells, MSCs)免疫调控能力的差异。方法 炎症因子干扰素γ(interferon gamma, IFNγ)和肿瘤坏死因子α(tumor necrosis factor alpha, TNFα)可诱导MSCs高表达吲哚胺-2,3-双加氧酶(indole-amine-2,3-dioxygenase, IDO)和趋化因子,从而抑制抗CD3/CD28抗体激活的T细胞增殖。通过RT-PCR及Western印迹法检测抑制T细胞增殖相关基因表达。使用Cell Tracer Violet的染料对T细胞进行标记,检测其增殖能力变化。同时,使用EdU染色检测MSCs细胞增殖。过程炎症因子刺激后的MSCs与PBMC进行共培养,检测PBMC的增殖情况,同时检测MSCs相关基因表达及其增殖情况。结果 炎症因子刺激的MSCs对抗CD3/28抗体激活的单个核细胞增殖有明显抑制作用,不同组织来源MSCs对单个核细胞增殖的抑制作用无明显区别。结论 与骨髓来源MSCs相比,其他组织来源MSCs对单个核细胞免疫调节作用无明显区别。

Abstract:

Objective To compare the immunomodulatory properties of human mesenchymal stem cells (MSCs) from different tissues. Methods Human adipose tissue-derived MSCs, umbilical cord-derived MSCs, bone marrow-derived MSCs, dental pulp-derived MSCs and skin-derived MSCs were treated with IFNγ and TNFα, then co-cultured with peripheral blood mononuclear cells (PBMCs). The proliferation of PBMCs was detected; and gene expression of PBMCs and MSCs was detected by RT-PCR and Western blotting. Results The proliferation of PBMCs co-cultured with stimulated MSCs was significantly inhibited. There were no significant differences in the inhibiting effects on PBMCs among MSCs from five different tissues. Conclusion MSCs derived from human adipose, umbilical cord, bone marrow, dental pulp and skin have no differences in immunomodulatory effect on mononuclear cells.