哮喘急性发作患者血清中IL-18、IL-33表达意义及其与肺功能的关系

Serum IL-18 and IL-33 levels in patients with acute asthma attack and its relationship with pulmonary function

关键词:  哮喘急性发作  支气管哮喘  IL-18  IL-33  临床意义

Key words:  asthma acute attack  bronchial asthma  IL-18  IL-33  clinical significance

摘要:

目的 探讨IL-33、IL-18表达水平与支气管哮喘急性发作之间的相关性。方法 选取上海普陀中心医院2014年5月至2014年11月收治的80例哮喘急性发作患者为哮喘观察组研究对象,同时选取同期来我院常规健康体检的80例健康体检者对照组研究对象。采用酶联免疫吸附法(ELISA)测定所有研究对象血清中IL-33和IL-18水平并做比较。78例哮喘患者康复出院后2周复查肺功能测定,比较患者住院治疗时肺功能并分析其与IL-18、IL-33相关性。结果哮喘组患者治疗前IL-18水平为(310.2±152.6)pg/ml,IL-33水平为(215.4±87.5)pg/ml,较健康组(204.2±95.4)pg/ml、(138.7±48.3)pg/ml偏高,差异具有统计学意义(P<0.05);哮喘组患者治疗后血清中IL-18水平为(215.5±98.2)pg/ml,IL-33水平为(146.6±54.9)pg/ml,较治疗前降低,差异具有统计学意义(P<0.05);健康组肺功能检查: FEV₁、FEV₁% pred和FEF50%值优于哮喘组,治疗后哮喘组FEV₁、FEV₁% pred和FEF50%等值优于治疗前,且经线性回归分析,IL-18和IL-33与FEV₁% pred等肺功能指标呈负性相关关系。结论哮喘急性发作患者血清中IL-18与IL-33表达水平较正常人高,治疗后两者水平均明显下降。两者与肺功能呈负相关。

Abstract:

Objective To investigate the serum levels of interleukin-33 (IL-33) and interleukin-18 (IL-18) in patients with acute asthma attack, and their relationship with pulmonary function of patients. Methods Eighty patients with acute asthma attack admitted in Shanghai Putuo Hospital from May 2014 to November 2014 were enrolled, and 80 healthy subjects were selected as control group. IL-33 and IL-18 levels were determined by enzyme-linked immunosorbent assay (ELISA). After treatment, pulmonary function tests were performed in 78 patients, and the correlation of IL-33 and IL-18 levels with lung function was analyzed. Results Serum IL-18 level [(310.2±152.6)pg/ml] and IL-33 level [(215.4±87.5)pg/ml] in asthma patients before treatment were higher than those in control group [(204.2±95.4)pg/ml and(138.7±48.3)pg/ml, P<0.05]. Compared to pretreatment levels, the IL-18 [(215.5±98.2)pg/ml] and IL-33 [(146.6±54.9)pg/ml] in asthma patients were significantly decreased (P<0.05) after treatment. The values of FEV₁, FEV₁% pred and FEF% in pulmonary function test of control group were better than those of asthma patients; while in asthma patients all these indicators were significantly improved after treatment. The linear regression analysis showed that serum IL-18 and IL-33 levels were negatively correlated with lung function. Conclusion Serum IL-18 and IL-33 levels are increased in patients with acute asthma attack, and are decreased after treatment; and IL-18 and IL-33 levels are negatively correlated with the pulmonary function of asthma patients.