健康成人脂蛋白相关磷脂酶A2和髓过氧化物酶质量浓度参考区间的研究

Study on reference interval of lipoprotein-associated phospholipase A2 and myeloperoxidase quality concentration in healthy adults

关键词:  脂蛋白相关磷脂酶A2  髓过氧化物酶  参考区间

Key words:  lipoprotein-associated phospholipase A2  myeloperoxidase  reference interval

摘要:

目的　建立中国健康人群脂蛋白相关磷脂酶A2（lipoprotein-associated phospholipase A2, Lp-PLA2）和髓过氧化物酶（myeloperoxidase, MPO）的正常参考区间。方法　入选5 367名（男2 534名，女2 833名）健康成人，分别按年龄段分成六组： 20~29岁、30~39岁、40~49岁、50~59岁、60~69岁和≥70岁组，ElISA法测定血浆Lp-PLA2和MPO质量水平。参考区间根据美国临床和实验室标准协会（CLSI）C28-A2的标准建立。结果　男性LP-PLA2水平显著高于女性（P＜005）；女性组Lp-PLA2水平有明显的年龄依赖性升高趋势（P＜005），其中≥60岁组LP-PLA2水平显著高于＜60岁组（P＜005）。而在男性的各年龄亚组中这种趋势差异无统计学意义（P＞005）。男女两组及各年龄亚组间MPO水平差异均无统计学意义（P＞005）。中国健康成人Lp-PLA2及MPO水平参考区间分别为≤19070 ng/mL、≤9868 ng/mL。结论　在中国健康成人中建立两项指标的参考区间，将有助于指导临床筛查易感人群心脑血管意外，评估高危患者的危险性，进行早期干预，从而有效降低急性心肌梗死和中风的发生率。

Abstract:

Objective　To establish the normal reference intervals of lipoprotein-associated phos-pholipase A2（Lp-PLA2) and myeloperoxidase（MPO) in Chinese healthy adults. Methods　A total of 5 367 healthy adults（2 534 males and 2 833 females) were recruited in the study. There were six age groups： 20-29, 30-39, 40-49, 50-59, 60-69 and over 70 years. Plasma Lp-PLA2 and MPO quality levels were assayed by ELISA. The reference intervals were established according to the criteria of the American Clinical and Laboratory Standards Institute（CLSI) C28-A2. Results　Lp-PLA2 levels in male were significantly higher than that in female（P<005). Lp-PLA2 levels had a significant trend in age-dependent increase in female（P<005), and the level in women over 60 years was significantly higher than that in women under 60 years（P<005). However, there was not significant difference in Lp-PLA2 levels among males of all age groups（P>005). There were no significant differences in MPO levels between males and females and among different age groups（P>005). The reference intervals of Lp-PLA2 and MPO levels in healthy Chinese adults were ≤19070 ng/mL and ≤9868 ng/mL respectively. Conclusion　The reference intervals of plasma Lp-PLA2 and MPO quality levels for healthy Chinese adults have been established in the study, which can be used for screening of susceptible population, evaluating the high-risk patients to carry out early intervention of cardiovascular and cerebrovascular diseases.