| 引用本文： |
-
华铮,姜洋,闫嘉乐,等.miR-122在PM2.5诱导的肺损伤中的作用机制[J].同济大学学报（医学版）,2024,45(2):170-175. [点击复制]
- HUA Zheng,JIANG Yang,YAN Jiale,et al.Roles of miR-122 in PM2.5-induced lung injury and its mechanism[J].Journal of Tongji University(Medical Science),2024,45(2):170-175. [点击复制]
|
|
| 摘要: |
| 目的探索PM2.5引起的肺损伤的分子机制，以期为肺损伤的诊疗提供新的靶点。
方法对PM2.5诱导下肺损伤的多组学数据进行差异基因筛选及分子功能分析，确定肺损伤相关的分子靶标，构建PM2.5诱导的SD大鼠肺损伤模型并进行初步验证。
结果HE染色结果显示，暴露组的大鼠肺泡间隔增宽，肺泡腔增大，部分肺泡断裂、融合；Masson染色结果发现暴露组大鼠肺部呈现出少量纤维化。本次实验结果发现，在PM2.5诱导条件下，miR-122表达显著上调，其靶基因胰岛素样生长因子1受体(insulin-like growth factor 1, IGF1R)表达显著下调。
结论PM2.5会导致大鼠的肺损伤，推测miR-122通过调控其靶基因IGF1R进而影响PI3K-AKT信号通路并引起细胞凋亡，加剧肺损伤。 |
| 关键词: PM2.5 肺损伤 miR-122 胰岛素样生长因子1受体 大鼠 |
| DOI：10.12289/j.issn.2097-4345.23169 |
| 通信作者: |
| 投稿时间：2023-05-22 |
| 录用日期： |
| 基金项目:上海市卫生健康委员会卫生行业临床研究专项（201940306）；浙江省基础公益计划项目(LGF22H010016) |
|
| Roles of miR-122 in PM2.5-induced lung injury and its mechanism |
| HUA Zheng,JIANG Yang,YAN Jiale,ZHANG Junfang,JIA Jingyi,ZHANG Jie1 |
| (School of Medical, Tongji University, Shanghai 200092, China;Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310012, China) |
| Abstract: |
| ObjectiveTo explore the molecular mechanism of PM2.5 induced lung injury in order to provide a new target for the diagnosis and treatment of lung injury.
MethodsDifferential expression analysis and molecular function analysis were performed on the multi-omics data to identify the molecular targets related to PM2.5-induced lung injury. PM2.5-induced lung injury model was established in SD rats, and the molecular targets related to lung injury was validated.
ResultsHE staining showed that the alveolar interval was widened, the alveolar cavity was enlarged, and part of the alveolar was broken and fused in the rats exposed to PM2.5. Masson staining showed a small amount of fibrosis in the lungs of exposed rats. Results showed that over-expression of miR-122 significantly down-regulated the expression of its target gene IGF1R under PM2.5 induction.
ConclusionThe study indicates that miR-122 may affect the PI3K-AKT signaling pathway and induce apoptosis by regulating IGF1R to aggravate lung injury in rats exposed to PM2.5. |
| Key words: PM2.5 lung injury miR-122 insulin-like growth factor 1 rat |
