维生素E对肿瘤坏死因子损伤 主动脉内皮细胞的防治作用

Rolesof Vitamin E on the Protection against Aortic EndothelialCell Injury Induced by Tumor Necrosis Factor in vitro

关键词:  维生素E  内皮细胞  肿瘤坏死因子  细胞增殖  细胞凋亡

摘要:

Objective　To explore the possible mechanism of vitamin E against atherosclerosis through its preventive effect on tumor necrosis factor (TNF) inducing endothelial cell injury.Methods　Bovine aortic endothelial cells were incubated in culture media with TNF-alpha (400 U/ml) and vitamin E (12.5 microM, 25.0 microM and 50.0 microM respectively) for 72 h.The morphology, proliferation, apoptosis and adherence of endothelial cells were investigated with phase-contrast microscope, MTT method, flow cytometry analysis and cell count respectively.Results　Compared with TNF group, the phenomena of endothelial cell deformity and shedding were slighter, the number of adhered HL60 monocyte cells and G₁-phase cells were less, and the inhibitory rate and the apoptotic peaks were significantly lower in vitamin E groups.Conclusion　The results suggested that vitamin E has definite protective effects on injury induced by TNF as shown in morphology, proliferation and apoptosis of endothelial cells in vitro.

Abstract: