| 引用本文： |
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崔大江,杨渭临,李秀丽.太白楤木总皂苷体外对乙肝病毒HBsAg、HBeAg的影响[J].同济大学学报（医学版）,2012,33(2):47-49. [点击复制]
- CUI Da-jiang,YANG Wei-lin,LI Xiu-li.Inhibitory effects of total saponin extracted from Aralia chinesis L on hepatitis virus B in vitro[J].Journal of Tongji University(Medical Science),2012,33(2):47-49. [点击复制]
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| 摘要: |
| 目的观察太白楤木总皂苷体外对乙肝病毒HBsAg和HBeAg的影响。方法以HepG 2215细胞为靶细胞,分别用不同浓度的太白楤木总皂苷和拉米夫定培养液培养,采用ELISA法测定细胞培养上清HBsAg、HBeAg 含量,评价太白楤木总皂苷对体外HBV的抑制作用。结果太白楤木总皂苷对体外培养HepG 2215细胞分泌的HBsAg、HBeAg的50％抑制浓度(IC50)分别为0.1、0.09 g/L,治疗指数(therapeutic index TI)分别为6.12、7.51.0.25g/L拉米夫定组和0.4 g/L太白楤木组在第3、6天对体外培养HepG 2215细胞分泌的HBsAg、HBeAg抑制作用差异无统计学意义(P＞0.05)。结论太白楤木总皂苷具有一定的体外抗HBV作用。 |
| 关键词: 太白楤木 HepG 2215细胞 肝炎病毒,乙型 |
| DOI：10.3969/j.issn1008-0392.2012.02.010 |
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| 基金项目:陕西省科学技术研究发展计划如05k16-G(3)] |
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| Inhibitory effects of total saponin extracted from Aralia chinesis L on hepatitis virus B in vitro |
| CUI Da-jiang,YANG Wei-lin,LI Xiu-li |
| (Dept.of Gereology,the Second Affiliated Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an 710004,China) |
| Abstract: |
| Objective To investigate the inhibitory effects of total saponin extracted from Aralia chinesis L.on hepatitis B virus in vitro.Methods HepG 2215 cells as target cells were exposed to different concentrations of total saponin of Aralia chinesis L.or lamivudine.Enzyme-linked immunosorbent assay was used for determining the HBsAg and HBeAg in supernatants of cell culture.Results The 50% inhibitory concentration(IC50) of total saponin on HBsAg and HBeAg production was 0.1 and 0.09g/L,respectively;therapeutic index was 6.12,7.51 respectively.There were no significant differences in inhibitory effects on HBsAg and HBeAg production between 0.4g/L of total saponin and 0.25g/L of lamivudine at d3 and d6 of treatment.Conclusion The total saponin extracted from Aralia chinesis L has inhibitory effects on HBV replication in vitro to some extent. |
| Key words: Aralia Chinesis Saponis HepG 2215 cell hepatitis B virus |
