LIU Jing,SUN Jie,CAO Hao,et al.Effects of magnetic silica nanospheres on the viability of human umbilical cord mesenchymal stem cells[J].Journal of Tongji University(Medical Science),2021,42(2):172-178. [点击复制]
Effects of magnetic silica nanospheres on the viability of human umbilical cord mesenchymal stem cells
LIU Jing,SUN Jie,CAO Hao,MEI Tian-xiao,ZHANG Yi-fan,LE Wen-jun,LIU Zhong-min
(Institute for Regenerative Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200123, China; Dept. of Burn & Plastic Surgery, Beijing Childrens Hospital, Capital Medical University, Beijing 100045, China; Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200123, China; 4. Shanghai Blood Center, Shanghai 200051, China;Institute for Regenerative Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200123, China; Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200123, China;Dept. of Cardiovascular Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China;Institute for Regenerative Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200124, China)
Abstract:
ObjectiveTo investigate the effect of magnetic silica nanospheres (MSNPs) on the viability of human umbilical cord mesenchymal stem cells (UCMSCs). MethodsMSNPs with high biocompatibility were prepared with solvothermal method and Stober method. The UCMSCs were isolated and cultured with tissue block culture method. The basic characteristics of the stem cells were observed and identified with microscopy, flow cytometry and three-line differentiation. The UCMSCs were incubated with MSNPs at different concentrations (1,10,50,100,200 μg/mL), and the MSNP-labeled stem cells were obtained by magnetic separation. The morphology, expression of stemness markers, proliferation and migration ability of MSNP-labeled stem cells were evaluated with fluorescence inverted microscopy, flow cytometry, CCK-8 detection, and cell scratch assay, respectively. ResultsThe UCMSCs with the basic characteristics of stem cells were successfully isolated and cultured. Microscopy showed that UCMSCs were labeled by different mass concentrations of MSNPs. CCK-8 test showed that 1 μg/mL microspheres promoted the proliferation of stem cells, while its inhibitory effect was enhanced with the increasing concentrations of MSNPs (10,50,100,200 μg/mL). Flow cytometry and cell migration assay showed that 10 μg/mL MSNPs did not significantly affect surface marker expression and migration of cells. ConclusionMSNPs can be used to label UCMSCs, without significant interfering with their active functions, such as stemness marker expression, cell proliferation and migration at low dose.
